Lab Research Analyst I for Neurobiology Duke University for School of Medicine United States

Req ID: 3468
Date: Aug 16, 2019
Location: Durham, NC, US, 27710
Personnel Area: MEDICAL CENTER
School of Medicine:

Established in 1930, Duke University School of Medicine is the youngest of the nation’s top medical schools. Ranked tenth among its peers, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where traditional barriers are low, interdisciplinary collaboration is embraced, and great ideas accelerate translation of fundamental scientific discoveries to improve humanhealth locally and around the globe.

Comprised of 2,400 faculty physicians and researchers, the Duke University School of Medicine along with the Duke University School of Nursing and Duke University Health System create Duke Health. Duke Health is a world-class health care network. Founded in 1998 to provide efficient, responsive care, the health system offers a full network of health services and encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Private Diagnostic Clinic, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.

Research Analyst I position at Duke Viral Vector Core

The Duke Viral Vector Core is a state-of-the art facility that provides comprehensive set of viral services to the Department of Neurobiology and Duke research community. We are currently looking for a Research Analyst I, who will help us to support ongoing service and research programs.

Essential Duties and Responsibilities

Assume responsibilities for production, purification, preparation and QC evaluation of AAV vectors. Contribute to production of other vector platforms including but not limited to retroviral vectors, lentiviral vectors and adenoviruses. Assist in general service operations of the Duke VVC.

Design and exercise various strategies for engineering and cloning new viral constructs, aimed to enhance packaging and expressional characteristics of adeno-associated and lentiviral-based vector systems. Generate/expand a collection of DREADDs and other optogenetic-expressing constructs and vectors.

Lead project aimed to develop novel gene-editing viral vector tools for high-throughput knockout of human genes.

Carry out molecular cloning and DNA manipulation, and additional molecular biology tasks to support ongoing service and research programs of the core.

Communicate effectively with the PI and other members of the team. The person will establish work flow with the help of principle investigator and expected to execute procedures and experiments independently with minimal supervision. The person will be expected to supervise and help the work of lower level employees, such as students and train new personnel.

Preferences, Education/Experience

Preferences:
Bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.
Demonstrated, extensive experience with molecular biology techniques is required, including: PCR, restriction digestion & cloning, primer design, plasmid isolation and analysis, bacterial transformation and screening, extraction of genomic DNA, DNA sequencing and analysis.
Accompanying data analysis software proficiency is required.
An extensive experience with production of AAVs.
The production and manipulation with other viral delivery platforms (lentiviral vectors, retroviral vectors, adenoviruses and vectors and HSV-1 viruses) is a preferred.

Minimum Qualifications

Education
Work requires a bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.

Experience
Work requires two years of research experience. A related master's degree may offset required years of experience on a 1:1 basis, e.g., a two year master's degree in lieu of two years of experience. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE

Duke University is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.

Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.

Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essential job functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.

Nearest Major Market: Durham
Nearest Secondary Market: Raleigh


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